Proteomic and Biochemical Evidence Involving Root Cell Wall Biosynthesis and Modification, Tricarboxylic Acid Cycle, and Glutathione Metabolism in Cultivar-Dependent Cd Accumulation of Water Spinach (Ipomoea aquatica).

Proteomic analysis and biochemical tests were employed to investigate the critical biological processes responsible for the different cadmium (Cd) accumulations between two water spinach (Ipomoea aquatica) cultivars, QLQ and T308. QLQ, with lower shoot Cd accumulation and translocation factor than T308, possessed higher expression of cell wall biosynthesis and modification proteins in roots, together with higher lignin and pectin contents, higher pectin methylesterase activity, and lower pectin methylation. The results demonstrated that QLQ could more effectively restrict root-to-shoot Cd translocation by compartmentalizing more Cd in root cell walls. In contrast, T308 showed higher expression of the tricarboxylic acid (TCA) cycle, glutathione (GSH) metabolism, and heavy metal transporter proteins, accompanied by higher GSH content and glutathione S-transferase (GST) and glutathione reductase (GR) activity, which accelerated Cd uptake and translocation in T308. These findings revealed several critical biological processes responsible for cultivar-dependent Cd accumulation in water spinach, which are important for elucidating Cd accumulation and transport mechanisms in different cultivars.

A novel microRNA IamiR-4-3p from water spinach (Ipomoea aquatica Forsk.) increased Cd uptake and translocation in Arabidopsis thaliana.

MicroRNAs (miRNAs) play important roles in plant response to Cd stress. In our previous study, we observed significant differences in the expression levels of IamiR-4-3p between high-Cd and low-Cd cultivars of water spinach. The function of IamiR-4-3p was investigated by using wild type Arabidopsis (WT), Arabidopsis transfected with empty vector pCambia1302 (CK), and Arabidopsis transfected with IamiR-4-3p + vector pCambia1302 (p35S::miR-4-3p) in this study. In p35S::miR-4-3p Arabidopsis, the expression levels of GST3 and AWPM19-like were reduced by 20% and 24%. Under Cd treatment, higher root and shoot Cd concentrations were detected in the transgenic p35S::miR-4-3p Arabidopsis. MDA and H2O2 concentrations were positively correlated with the Cd concentrations in all Arabidopsis. The elevated GSH pool in p35S::miR-4-3p Arabidopsis should compensate for its restricted GST3 expression in response to Cd-induced oxidative stress. Lower F1 (cell wall) and higher F2 (organelle) and F3 (soluble fraction) Cd concentrations were observed along with the reduced ABA level in p35S::miR-4-3p Arabidopsis, which could induce a weakened apoplastic barrier and higher Cd accumulation and translocation in roots. It is suggested that IamiR-4-3p is able to reduce the expression levels of GST3 and AWPM19-like, resulting in higher Cd uptake and translocation in Arabidopsis.

Characterization of cadmium accumulation mechanism between eggplant (Solanum melongena L.) cultivars.

Excessive cadmium (Cd) accumulation in vegetables due to farmland pollution constitutes a serious threat to human health. Eggplant has a tendency to accumulate Cd. To investigate the mechanism of the differences in Cd accumulation levels between high-Cd (BXGZ) and low-Cd (MYQZ) eggplant cultivar, physiological and biochemical indicators and mRNA expression of eggplant were examined using photosynthetic apparatus, biochemical test kits, Fourier transform infrared (FTIR) spectroscopy and transcriptome sequencing, etc. The results of biochemical test kits and FTIR revealed that MYQZ enhanced pectin methylesterase (PME) activity, and lignin and pectin content in the root cell wall, which was associated with the upregulation of PME, cinnamyl-alcohol dehydrogenase and peroxidase (PODs). Higher levels of cysteine and glutathione (GSH) contents and upregulation of genes associated with sulfur metabolism, as well as higher expression of ATP-binding cassette transporters (ABCs), cation exchangers (CAX) and metal tolerance proteins (MTPs) were observed in MYQZ. In BXGZ, the higher stomatal density and stomatal aperture as well as higher levels of Ca2+ binding protein-1 (PCaP1) and aquaporins and lower levels of A2-type cyclins (CYCA2-1) are consistent with an enhanced transpiration rate in BXGZ. Furthermore, a more developed root system was shown to be associated with higher levels of auxin response factor (ARF19), GATA transcription factors (GATA4, 5 and 11) and auxin efflux carrier component (PIN5) in BXGZ. In conclusion, highly active PME, and higher levels of lignin and pectin in MYQZ are expected to reduce Cd toxicity, while Cd translocation can be inhibited with the help of ABC and other Cd transporters. As for BXGZ, the uptake and translocation of Cd were enhanced by the developed root system and stronger transpiration.

Molecular and biochemical mechanisms underlying boron-induced alleviation of cadmium toxicity in rice seedlings.

Both cadmium (Cd) contamination and boron (B) deficiency in farmland soils pose a threat to the yield and quality of crops in Southern China. The present study investigated the mechanisms by which B reduces Cd accumulation in rice (Oryza sativa) seedlings. Boron supplementation partially restored the decline in shoot and root biomass caused by Cd treatment (26% and 33%, respectively), with no significant difference between the B+Cd and control groups. We also found that B significantly reduced shoot and root Cd concentrations (by 64% and 25%, respectively) but increased Cd concentration (by 43%) and proportion (from 38% to 55%) in root cell walls. Transcriptome analysis and biochemical tests suggested that B supplementation enhanced lignin and pectin biosynthesis, pectin demethylation, and sulfur and glutathione metabolism. Moreover, B decreased the expression of some Cd-induced transporter-related genes (i.e., HMA2, Nramp1, and several ABC genes). These results indicate that B relieved Cd toxicity and reduced Cd accumulation in rice seedlings by restraining Cd uptake and translocation from root to shoot by improving Cd tolerance and chelation ability. These novel findings would benefit further investigations into how B influences Cd uptake, translocation, detoxification, and accumulation in crops.

Transcriptome analysis and physiological indicators reveal the role of sulfur in cadmium accumulation and transportation in water spinach (Ipomoea aquatica Forsk.).

Cadmium (Cd) contamination of croplands has become a threat to crop food safety and human health. In this study, we investigated the effect of sulfur on the growth of water spinach under Cd stress and the amount of Cd accumulation by increasing the soil sulfate content. We found that the biomass of water spinach significantly increased after the application of sulfur while the shoot Cd concentration was considerably reduced (by 31%). The results revealed that sulfur could promote the expression of PME and LAC genes, accompanied by an increase in PME activity and lignin content. Also, the cell wall Cd content of water spinach roots was significantly increased under sulfur treatment. This finding suggests that sulfur could enhance the adsorption capacity of Cd by promoting the generation of cell wall components, thereby inhibiting the transportation of Cd via the apoplastic pathway. In addition, the higher expression of Nramp5 under the Cd1S0 (concentration of Cd and sulfur are 2.58 and 101.31 mg/kg respectively) treatment led to increased Cd uptake. The CAX3 and ABC transporters and GST were expressed at higher levels along with a higher cysteine content and GSH/GSSR value under Cd1S1 (concentration of Cd and sulfur are 2.60 and 198.36 mg/kg respectively) treatment, which contribute to the Cd detoxification and promotion of Cd compartmentalization in root vacuoles, thereby reducing the translocation of Cd to the shoot via the symplastic pathway.

Selection for low-cadmium cultivars and cadmium subcellular distribution comparison between two selected cultivars of eggplant (Solanum melongena L.).

Excessive accumulation of cadmium (Cd) in vegetables poses a serious threat to human health; therefore, it is urgent to screen and cultivate vegetable cultivars with low Cd accumulation in the edible parts. Eggplant has a high tendency for Cd accumulation, but research on its low Cd accumulation cultivars is still rare. In this study, to screen low-Cd cultivars, 30 eggplant cultivars were screened using soils containing 0.22 mg/kg, 2.9 mg/kg (low-Cd), and 4.7 mg/kg of Cd (high-Cd). MYCQ and ZGQ were confirmed as low-Cd cultivars, BXGZ and WCCQ were confirmed as high-Cd cultivars, and a 2.52-3.88-fold difference in Cd concentration was observed in their fruits. The subcellular distribution revealed that the root cell wall and vacuole Cd concentrations of a typical low-Cd cultivar (MYCQ) were significantly higher than those of a typical high-Cd cultivar (BXGZ); however, the Cd concentrations in the cell wall and vacuole in fruits, leaves, and stems were significantly lower in MYCQ than in BXGZ. These results indicated that the low-Cd cultivars of eggplant could lessen Cd toxicity through the elevated Cd retention and sequestration levels of root cell walls and vacuoles, thus reducing Cd transport from roots to aboveground tissues, leading to low Cd accumulation. The findings of this study can provide a physiological and biochemical foundation for the screening and breeding of low-Cd cultivars of fruit vegetables and demonstrates that the application of low-Cd cultivars is necessary for food safety in humans.